SYNTHESIS AND PURIFICATION OF [1-P-32]FRUCTOSE-1,6-BISPHOSPHATE WITH HIGH SPECIFIC RADIOACTIVITY

被引：17

作者：

MCCLARD, RW ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT CHEM,LOS ANGELES,CA 90024

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90612-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The straightforward, rapid synthesis and purification of radiochemically pure [1-32P]fructose-1,6-bisphosphate starting from radioactively labeled inorganic phosphate is described. The product has a relatively high specific radioactivity (2 × 105 Ci/mol). Using this method, carrier-free [1-32P]fructose-1,6-bisphosphate could be obtained. The use of [1-32P]fructose-1,6-bisphosphate in the assay of fructose bisphosphatase is illustrated. © 1979.

引用

页码：500 / 503

页数：4

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