CONSTRUCTION AND REPLICATION PROPERTIES OF HYBRID PLASMIDS COMPOSED OF THE R-DETERMINANT OF R1001 AND THE PLASMIDS PCRI OR PSC201

被引:8
作者
LANE, D [1 ]
CHANDLER, M [1 ]
SILVER, L [1 ]
BRUSCHI, A [1 ]
CARO, L [1 ]
机构
[1] UNIV GENEVA,DEPT BIOL MOLEC,CH-1211 GENEVA 4,SWITZERLAND
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 03期
关键词
D O I
10.1007/BF00271505
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned the entire r-determinant of the antibiotic resistance plasmid R100.1 on the plasmid vectors pCR1 and pSC201. We find that the hybrid plasmids segregate from cultures in which replication of the vector is blocked. This suggests that the r-det is not capable of autonomous replication. © 1979 Springer-Verlag.
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页码:337 / 340
页数:4
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