STABILITY OF METHYLATED PURINES AND OF METHYLPHOSPHOTRIESTERS IN THE DNA OF V79-CELLS AFTER TREATMENT WITH N-METHYL-N-NITROSOUREA

被引：92

作者：

WARREN, W ^{[1
]}

CRATHORN, AR ^{[1
]}

SHOOTER, KV ^{[1
]}

机构：

[1] ROYAL CANC HOSP,POLLARDS WOOD RES STN,CHALFONT ST GILES HP8 4SP,BUCKINGHAMSHIRE,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 563卷 / 01期

基金：

英国医学研究理事会;

关键词：

(V79 cell); DNA repair; Methyl nitrosourea; Methyl phosphotriester; Methylated purine;

D O I：

10.1016/0005-2787(79)90009-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

V79-379A cells growing in suspension culture were treated with N-methyl-N-nitrosourea at concentrations of 0.6 and 1.2 mM. After incubation for periods from 1 to 48 h DNA was isolated from the cells and the concentrations of 7-methylguanine, O6-methylguanine, 3-methyladenine and methyl phosphotriesters were determined. After correction for dilution resulting from DNA synthesis during the incubation it was found that no loss of O6-methylguanine or methylphosphotriesters occurred; 7-methylguanine disappeared with a half-life of 22 h and 3-methyladenine was detectable only immediately after the initial treatment. The results show that these cells eliminate 7-methylguanine and 3-methyladenine from DNA by a repair process but are unable to excise or repair O6-methylguanine or methyl phosphotriesters. © 1979.

引用

页码：82 / 88

页数：7

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