BINDING SHIFT ASSAY OF PARVALBUMIN, CALMODULIN AND CARBONIC-ANHYDRASE BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

被引：33

作者：

KAJIWARA, H

HIRANO, H

OONO, K

机构：

[1] National Institute of Agrobiological Resources, Tsukuba, Ibaraki

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 22卷 / 04期

关键词：

CALMODULIN; CAPILLARY ELECTROPHORESIS; CE; HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS; HPCE; CARBONIC ANHYDRASE; PARVALBUMIN;

D O I：

10.1016/0165-022X(91)90032-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Shifts in mobility caused by binding of Ca2+ to calmodulin and parvalbumin were studied using high-performance capillary electrophoresis in a Tris-glycine buffer, rather than conventional polyacrylamide gel electrophoresis which requires larger amounts of sample and longer assay time. A Zn2+-binding protein, carbonic anhydrase, also showed a partial shift in mobility following Zn2+-binding.

引用

页码：263 / 268

页数：6

共 10 条

[1] COUPLING CAPILLARY ZONE ELECTROPHORESIS AND CONTINUOUS-FLOW FAST ATOM BOMBARDMENT MASS-SPECTROMETRY FOR THE ANALYSIS OF PEPTIDE MIXTURES
CAPRIOLI, RM
MOORE, WT
MARTIN, M
DAGUE, BB
WILSON, K
MORING, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1989, 480 : 247 - 257
[2] Davis B.J., 1964, ANN NY ACAD SCI, V121, P321
[3] AMINO-ACID SEQUENCE OF PARVALBUMIN FROM RABBIT SKELETAL-MUSCLE
ENFIELD, DL
ERICSSON, LH
BLUM, HE
FISCHER, EH
NEURATH, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (04) : 1309 - 1313
[4] A SEMIEMPIRICAL MODEL FOR THE ELECTROPHORETIC MOBILITIES OF PEPTIDES IN FREE-SOLUTION CAPILLARY ELECTROPHORESIS
GROSSMAN, PD
COLBURN, JC
LAUER, HH
[J]. ANALYTICAL BIOCHEMISTRY, 1989, 179 (01) : 28 - 33
[5] APPLICATION OF FREE-SOLUTION CAPILLARY ELECTROPHORESIS TO THE ANALYTICAL SCALE SEPARATION OF PROTEINS AND PEPTIDES
GROSSMAN, PD
COLBURN, JC
LAUER, HH
NIELSEN, RG
RIGGIN, RM
SITTAMPALAM, GS
RICKARD, EC
[J]. ANALYTICAL CHEMISTRY, 1989, 61 (11) : 1186 - 1194
[6] KAKIUCHI S, 1981, J BIOL CHEM, V256, P19
[7] DETERMINATION OF THE COMPLETE AMINO-ACID-SEQUENCE OF CALMODULIN (PHENYLALANINE-RICH ACIDIC PROTEIN-II) FROM BOVINE BRAIN
KASAI, H
KATO, Y
ISOBE, T
KAWASAKI, H
OKUYAMA, T
[J]. BIOMEDICAL RESEARCH-TOKYO, 1980, 1 (03): : 248 - 264
[8] KLEE CB, 1980, CALCIUM CELL FUNCTIO, V1, P59
[9] STRUCTURE AND CATALYSIS OF ENZYMES
LIPSCOMB, WN
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 : 17 - 34
[10] A H-1-NMR DETERMINATION OF THE SOLUTION CONFORMATION OF A SYNTHETIC PEPTIDE ANALOG OF CALCIUM-BINDING SITE-III OF RABBIT SKELETAL TROPONIN-C
MARSDEN, BJ
HODGES, RS
SYKES, BD
[J]. BIOCHEMISTRY, 1989, 28 (22) : 8839 - 8847

← 1 →