LIGAND PROPERTIES OF DIETHYLSTILBESTROL - STUDIES WITH PURIFIED NATIVE AND FATTY ACID-FREE RAT ALPHA-1-FETOPROTEIN AND ALBUMIN

We report the equilibrium binding parameters for the interactions of the estrogen analogue diethylstilbestrol (DES) with highly purified rat alpha1-fetoprotein (AFP) and serum albumin preparations. At 25°C and pH 7.4, an association constant (Ka) of about 1.5 × 106M-1 and 2 sites/ mole are measured with the DES-AFP system, whereas for the DES-albumin interaction, we find a Ka of approximately 2 × 105M-1 and about 11 sites/mole of protein. The removal of fatty acids from pure AFP causes a reversible 3 fold increase of the number of DES binding sites; the same delipidation procedure applied to albumin slightly diminishes its DES binding parameters. We also demonstrate the capability of DES to displace competitively estradiol-17β (E2) from its high affinity sites on the estrophilic rat AFP. Finally, the binding behaviour of the two serum proteins towards the synthetic estrogen is compared to their interaction with the natural hormones. The physiological and pharmacological relevance of these data is discussed. © 1979.