TRANSCRIPTION EFFICIENCY OF HUMAN APOLIPOPROTEIN-A-I PROMOTER VARIES WITH NATURALLY-OCCURRING A-TO-G TRANSITION

被引：42

作者：

TUTEJA, R ^{[1
]}

TUTEJA, N ^{[1
]}

MELO, C ^{[1
]}

CASARI, G ^{[1
]}

BARALLE, FE ^{[1
]}

机构：

[1] PRASSIS SETTIMO MILANESE,I-20019 MILAN,ITALY

来源：

FEBS LETTERS | 1992年 / 304卷 / 01期

关键词：

APOLIPOPROTEIN-A-I; PROMOTER; CAT ASSAY; TRANSCRIPTION; HUMAN;

D O I：

10.1016/0014-5793(92)80597-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human, the gene coding for apolipoprotein A-I (apo A-I), a protein of the plasma lipid transport system, is expressed only in the liver and the intestine. A naturally occurring A to G substitution in the promoter at position -78 was shown to be associated with high density lipoproteins (HDL) in females. We have studied the effect of this mutation on promoter activity using various lengths of promoter sequences and the CAT reporter gene system. Transient expression studies after introduction of these constructs into Hep 3B cells revealed that in the region spanning -330 to + 1 of the promoter an A to G substitution increases the activity approximately twofold. On the other hand, when further upstream region (-1469 to +397) is also included, the promoter activity seems comparable in both alleles. Our results show how minimal sequence variations can affect the in vitro analysis of promoter activity.

引用

页码：98 / 101

页数：4

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