PRODUCTION OF NITRITE BY NEONATAL RAT MICROGLIAL CELLS BRAIN MACROPHAGES

被引：209

作者：

ZIELASEK, J

TAUSCH, M

TOYKA, KV

HARTUNG, HP

机构：

[1] Department of Neurology, University of Würzburg, D-8700 Würzburg

来源：

CELLULAR IMMUNOLOGY | 1992年 / 141卷 / 01期

关键词：

D O I：

10.1016/0008-8749(92)90131-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microglial cells/brain macrophages from neonatal rats were examined for their capacity to generate nitrite, a product of the NO pathway. Upon incubation with bacterial lipopolysaccharide (LPS) or rat interferon-γ (IFN-γ), cells from the microglia-enriched fraction released measurable amounts of nitrite into the supernatant within 24-48 hr. The production of nitrite was dependent on the cell number and the dose of IFN-γ and LPS. It could be inhibited by NG-monomethylarginine. We conclude that activated microglial cells can secrete nitrite. Stimulation of the NO pathway in microglial cells may be relevant to the pathogenesis of inflammatory and autoimmune demyelinating diseases of the brain. © 1992.

引用

页码：111 / 120

页数：10

共 30 条

[1] A SIMPLE MICROPLATE ASSAY FOR THE DETERMINATION OF CELLULAR PROTEIN [J].