DETECTION BY CAPILLARY ELECTROPHORESIS OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM - ANALYSIS OF A POLYMERASE CHAIN REACTION-AMPLIFIED PRODUCT OF THE DXS-164 LOCUS IN THE DYSTROPHIN GENE

Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.