SUBCELLULAR-DISTRIBUTION OF O-ACETYLSERINE(THIOL)LYASE IN CAULIFLOWER (BRASSICA-OLERACEA L) INFLORESCENCE

被引：49

作者：

ROLLAND, N ^{[1
]}

DROUX, M ^{[1
]}

DOUCE, R ^{[1
]}

机构：

[1] RHONE POULENC AGROCHIM, CNRS,UM 41,UNITE MIXTE CNRS RHONE POULENC, 14-20 IMPASSE PIERRE BAIZET, F-69263 LYONS 09, FRANCE

来源：

PLANT PHYSIOLOGY | 1992年 / 98卷 / 03期

关键词：

D O I：

10.1104/pp.98.3.927

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The subcellular localization of O-acetylserine(thiol)lyase (EC 4.2.99.8) in nongreen tissue from higher plants has been studied using purified proplastids, mitochondria, and protoplasts from cauliflower (Brassica oleracea L.) buds as a source of subcellular fractions. O-Acetylserine(thiol)lyase has been detected in both organelles (proplastids and mitochondria) and a cytosolic extract obtained by protoplast fractionation. We confirmed these observations, demonstrating that a form of the enzyme different in global charge and separated from others by anion-exchange chromatography corresponded to each subcellular location. Our observations are consistent with the need for cysteine biosynthesis in each subcellular compartment where the synthesis of proteins occurs.

引用

页码：927 / 935

页数：9

共 35 条

[1] PREPARATION AND CHARACTERIZATION OF ENVELOPE MEMBRANES FROM NONGREEN PLASTIDS [J].