A FAST AND SIMPLE METHOD TO DETERMINE THE CLONAL RELATIONSHIP AMONG HUMAN T-CELL LYMPHOCYTES

被引：22

作者：

DEBOER, JG

CURRY, JD

GLICKMAN, BW

机构：

[1] Biology Department, Center for Environmental Health, University of Victoria, Victoria

来源：

MUTATION RESEARCH | 1993年 / 288卷 / 01期

基金：

英国医学研究理事会;

关键词：

HUMAN T-CELL LYMPHOCYTES; LYMPHOCYTES; HUMAN T-CELL; CLONAL RELATIONSHIP; DETERMINATION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0027-5107(93)90218-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

For the analysis,of mutation in human T-cell lymphocytes, it is crucial 'to determine the clonal relationship between isolated mutants, particularly if they harbour identical mutations. Here we report an efficient method to determine the clonal relationships between these cells. The method is based on the analysis of restriction fragment length polymorphisms of a polymerase chain reaction amplified, rearranged T-cell receptor gamma-gene. As few as 600 cells are sufficient, regular agarose gels can be used for the separation of the restriction fragments, no radioactive label is required, and results can be obtained in 2 days.

引用

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页码：173 / 180

页数：8

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