STRUCTURE AND DIFFERENTIAL RESPONSE TO ABSCISIC-ACID OF 2 PROMOTERS FOR THE CYTOSOLIC COPPER ZINC-SUPEROXIDE DISMUTASE GENES, SODCC1 AND SODCC2, IN RICE PROTOPLASTS

被引：58

作者：

SAKAMOTO, A ^{[1
]}

OKUMURA, T ^{[1
]}

KAMINAKA, H ^{[1
]}

SUMI, K ^{[1
]}

TANAKA, K ^{[1
]}

机构：

[1] KYOTO PREFECTURAL UNIV, COLL AGR, DEPT BIOCHEM, KYOTO 606, JAPAN

来源：

FEBS LETTERS | 1995年 / 358卷 / 01期

关键词：

ABSCISIC ACID; DIFFERENTIAL GENE REGULATION; INDUCIBLE PROMOTER; SUPEROXIDE DISMUTASE; CELLULAR REDOX; RICE;

D O I：

10.1016/0014-5793(94)01396-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the 5'-flanking sequences of two nuclear genes (SodCc1 and SodCc2) encoding cytosolic copper/zinc-superoxide dismutase in rice (Oryza sativa L.). Utilizing transient P-glucuronidase (GUS) reporter assays, functional promoter-GUS analysis was performed in rice protoplasts exposed to the phytohormone abscisic acid (ABA) or the antioxidant sulfhydryl reagent, dithiothreitol (DTT). Transcriptional activities from both SodCc-GUS fusions were stimulated by DTT, which induces the promoter activity of the tobacco SodCc gene [Proc. Natl. Acad. Sci. USA 90 (1993) 3108-3112]. ABA had no effect on SodCc1-GUS expression but specifically induced the gene expression of the SodCc2-GUS fusion. The simultaneous application of ABA and gibberellin A(3), however, abolished the enhancing effect of ABA. These results indicated that two rice SodCc promoters differentially respond to externally supplied ABA and that one of the regulatory factors for plant SodCc expression is ABA in addition to cellular redox-modulating antioxidants.

引用

页码：62 / 66

页数：5

共 26 条

[1]

Asada K, 1980, CHEM BIOCH ASPECTS S, P136

[2] SUPEROXIDE-DISMUTASE AND STRESS TOLERANCE [J].

BOWLER, C ;

VANMONTAGU, M ;

INZE, D .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :83-116

[3] THE INDUCTION OF MANGANESE SUPEROXIDE-DISMUTASE IN RESPONSE TO STRESS IN NICOTIANA-PLUMBAGINIFOLIA [J].

BOWLER, C ;

ALLIOTTE, T ;

DELOOSE, M ;

VANMONTAGU, M ;

INZE, D .

EMBO JOURNAL, 1989, 8 (01) :31-38

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] TRANSCRIPTIONAL REGULATION OF THE HEAT-SHOCK RESPONSE - A PLANT PERSPECTIVE [J].

GURLEY, WB ;

KEY, JL .

BIOCHEMISTRY, 1991, 30 (01) :1-12

[6] GENETIC-ENGINEERING OF OXIDATIVE STRESS RESISTANCE IN HIGHER-PLANTS [J].

HEROUART, D ;

BOWLER, C ;

WILLEKENS, H ;

VANCAMP, W ;

SLOOTEN, L ;

VANMONTAGU, M ;

INZE, D .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1993, 342 (1301) :235-240

[7] DEVELOPMENTAL AND ENVIRONMENTAL-REGULATION OF THE NICOTIANA-PLUMBAGINIFOLIA CYTOSOLIC CU/ZN-SUPEROXIDE DISMUTASE PROMOTER IN TRANSGENIC TOBACCO [J].

HEROUART, D ;

VANMONTAGU, M ;

INZE, D .

PLANT PHYSIOLOGY, 1994, 104 (03) :873-880

[8] REDOX-ACTIVATED EXPRESSION OF THE CYTOSOLIC COPPER-ZINC SUPEROXIDE-DISMUTASE GENE IN NICOTIANA [J].

HEROUART, D ;

VANMONTAGU, M ;

INZE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3108-3112

[9]

JEFFERSON RA, 1987, EMBO J, V6, P3901

[10]

KANEMATSU S, 1989, PLANT CELL PHYSIOL, V30, P381

← 1 2 3 →