INHIBITION OF PROLIFERATING LENS EPITHELIUM WITH ANTITRANSFERRIN RECEPTOR IMMUNOTOXIN

被引：21

作者：

GOINS, KM

ORTIZ, JR

FULCHER, SFA

HANDA, JT

JAFFE, GJ

FOULKS, GN

COBO, LM

机构：

[1] University of Chicago Medical Center, Chicago, Illinois

[2] Duke University Eye Center, Durham, North Carolina

来源：

JOURNAL OF CATARACT AND REFRACTIVE SURGERY | 1994年 / 20卷 / 05期

关键词：

ANTITRANSFERRIN RECEPTOR; IMMUNOTOXIN; INHIBITION; LENS EPITHELIUM; POSTERIOR CAPSULE OPACIFICATION;

D O I：

10.1016/S0886-3350(13)80230-1

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We investigated the effect of an antitransferrin receptor immunotoxin (454A12-rRA) on proliferating human and baboon lens epithelium in vitro. Human and baboon lens epithelial cells grown in modified TC-199 medium at 35 degrees Celsius in 7% CO2 were seeded in 24 well plates at a density of 17,500 cells/ml to 40,000 cells/ml. The cells were exposed to various concentrations of 454A12-rRA for seven days. The sensitivity of proliferating human lens epithelium to 454A12-rRA was dependent on the dose, with a 60% to 70% reduction in cell counts at immunotoxin concentrations of 100 ng/ml and above. The immunotoxin had no significant effect on baboon lens epithelium in vitro, which suggests that it is specific for human tissue. By preventing the proliferation of human lens epithelial cells, immunotoxin 454A12-rRA may be useful in the management of posterior capsule opacification after planned extracapsular cataract surgery.

引用

页码：513 / 516

页数：4

共 25 条

[1]

McDonnell PJ, Zarbin MA, Green WR, Posterior capsule opacification in pseudophakic eyes, Ophthalmology, 90, pp. 1548-1553, (1983)

[2]

Moisseiev J, Bartov E, Schochat A, Blumenthal M, Longterm study of the prevalence of capsular opacification following extracapsular cataract extraction, J Cataract Refract Surg, 15, pp. 531-533, (1989)

[3]

Ohadi C, Moriera H, McDonnell P, Posterior capsule opacification, Curr Opin Ophthalmol, 2, pp. 46-52, (1991)

[4]

Percival SPB, Setty SS, Analysis of the need for secondary capsulotomy during a five-year follow-up, J Cataract Refract Surg, 14, pp. 379-382, (1988)

[5]

Rickman-Barger L, Florine CW, Larson RS, Lindstrom RL, Retinal detachment after neodymium:YAG laser posterior capsulotomy, Am J Ophthalmol, 107, pp. 531-536, (1989)

[6]

Leff SR, Welch JC, Tasman W, Rhegmatogenous retinal detachment after YAG laser posterior capsulotomy, Ophthalmology, 94, pp. 1222-1225, (1987)

[7]

Winslow RL, Taylor BC, Retinal complications following YAG laser capsulotomy, Ophthalmology, 92, pp. 785-789, (1985)

[8]

Weinreb RN, Wasserstrom JP, Parker W, Neovascular glaucoma following neodymium-YAG laser posterior capsulotomy, Arch Ophthalmol, 104, pp. 730-731, (1986)

[9]

Apple DJ, Solomon KD, Tetz MR, Et al., Posterior capsule opacification, Surv Ophthalmol, 37, pp. 73-116, (1992)

[10]

Zaturinsky B, Naveh N, Saks D, Solomon AS, Prevention of posterior capsular opacification by cryolysis and the use of heparinized irrigating solution during extracapsular lens extraction in rabbits, Ophthalmic Surg, 21, pp. 431-434, (1990)

← 1 2 3 →