TRANSCRIPTION BY EUKARYOTIC RNA POLYMERASES-A AND POLYMERASES-B OF CHROMATIN ASSEMBLED INVITRO

被引：63

作者：

WASYLYK, B

CHAMBON, P

机构：

[1] Institut de Chimie Biologique, Faculté de Médecine, Université Louis-Pasteur, Strasbourg, F-67085

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13191.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin was assembled in vitro from simian virus 40 DNA form I and the calf‐thymus four histones H2A, H2B, H3 and H4. Transcription with calf thymus RNA polymerases A and B (I and II) was greatly inhibited. Nucleosomes were found to inhibit both RNA chain initiation and elongation. The inhibition of elongation could be relieved by increasing ionic strength, suggesting that electrostatic interactions between histone octamer and DNA have to be broken for RNA polymerase to transcribe DNA organised into nucleosomes. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：317 / 327

页数：11

共 56 条

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