A NEW METHOD FOR TOTAL PROTEIN DETERMINATION

被引：12

作者：

BARRETO, WJ ^{[1
]}

DEAQUINO, M ^{[1
]}

ZAIA, DAM ^{[1
]}

DEAQUINO, M ^{[1
]}

ZAIA, DAM ^{[1
]}

机构：

[1] UNIV ESTADUAL LONDRINA,CTR CIENCIAS EXATAS,DEPT QUIM,CAMPUS UNIV,BR-86051 LONDRINA,PARANA,BRAZIL

来源：

ANALYTICAL LETTERS | 1990年 / 23卷 / 07期

关键词：

milk; protein; quinone; spectrophotometry;

D O I：

10.1080/00032719008054353

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for total protein determination was developed and applied to samples of skim milk powder. The method is based on the products of the reaction between p-benzoquinone and protein (casein), which show a charge-transfer band at 350 nm. The effects of concentration, reaction time, and pH were studied. The results were compared with those obtained with the Kjeldahl and Lowry methods; the standard deviations observed with the proposed method and with Lowry’s method are of the same order of magnitude. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：1279 / 1290

页数：12

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