IMMUNOHISTOCHEMICAL DETECTION OF A MONOCLONAL-ANTIBODY DIRECTED AGAINST THE NGF RECEPTOR IN BASAL FOREBRAIN NEURONS FOLLOWING INTRAVENTRICULAR-INJECTION

被引:28
作者
THOMAS, LB
BOOK, AA
SCHWEITZER, JB
机构
[1] UNIV TENNESSEE,CTR HLTH SCI,DEPT PATHOL,DIV NEUROPATHOL,800 MADISON AVE,RM 568 BMH-M,MEMPHIS,TN 38163
[2] UNIV TENNESSEE,CTR HLTH SCI,DEPT ANAT & NEUROBIOL,MEMPHIS,TN 38163
关键词
NERVE GROWTH FACTOR RECEPTOR; CHOLINERGIC BASAL FOREBRAIN; IMMUNOHISTOCHEMISTRY; AUTORADIOGRAPHY; MONOCLONAL ANTIBODY; CEREBROSPINAL FLUID;
D O I
10.1016/0165-0270(91)90019-V
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
It has been shown by autoradiography that, following intraventricular administration, a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor is specifically accumulated bilaterally by numerous cholinergic neurons of the basal forebrain. This is consistent with the evidence that cholinergic basal forebrain neurons have NGF receptors and respond to NGF under a variety of experimental conditions. The present study demonstrates that the immunohistochemical detection of unmodified monoclonal antibody in cholinergic forebrain neurons following transport from CSF is feasible, although injection of larger amounts of the antibody is required to obtain an image equivalent to the one obtained with the autoradiographic method. The location of the immunohistochemical product clearly indicates that the antibody has been internalized, probably in an endosomal compartment.
引用
收藏
页码:37 / 45
页数:9
相关论文
共 24 条
[1]  
CHANDLER CE, 1984, J BIOL CHEM, V259, P6882
[2]   SELECTIVE DESTRUCTION OF NERVE GROWTH-FACTOR RECEPTOR BEARING CELLS-INVITRO USING A HYBRID TOXIN COMPOSED OF RICIN-A CHAIN AND A MONOCLONAL-ANTIBODY AGAINST THE NERVE GROWTH-FACTOR RECEPTOR [J].
DISTEFANO, PS ;
SCHWEITZER, JB ;
TANIUCHI, M ;
JOHNSON, EM .
JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :1107-1114
[3]  
GNAHN H, 1983, DEV BRAIN RES, V9, P45, DOI 10.1016/0165-3806(83)90107-4
[4]  
HEFTI F, 1986, J NEUROSCI, V6, P2155
[5]   CHRONIC INTRAVENTRICULAR-INJECTIONS OF NERVE GROWTH-FACTOR ELEVATE HIPPOCAMPAL CHOLINE-ACETYLTRANSFERASE ACTIVITY IN ADULT-RATS WITH PARTIAL SEPTO-HIPPOCAMPAL LESIONS [J].
HEFTI, F ;
DRAVID, A ;
HARTIKKA, J .
BRAIN RESEARCH, 1984, 293 (02) :305-311
[6]   OBSERVATIONS ON PASSAGE OF FLUORESCEIN LABELED SERUM PROTEINS ( FLSP ) FROM CEREBROSPINAL FLUID [J].
KLATZO, I ;
MIQUEL, J ;
SMITH, DE ;
PROKOP, JD ;
FERRIS, PJ .
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1964, 23 (01) :18-&
[7]   LOCALIZATION OF NERVE GROWTH-FACTOR RECEPTOR MESSENGER-RNA AND PROTEIN IN THE ADULT-RAT BRAIN [J].
KOH, S ;
OYLER, GA ;
HIGGINS, GA .
EXPERIMENTAL NEUROLOGY, 1989, 106 (03) :209-221
[8]   LEVELS OF NERVE GROWTH-FACTOR AND ITS MESSENGER-RNA IN THE CENTRAL NERVOUS-SYSTEM OF THE RAT CORRELATE WITH CHOLINERGIC INNERVATION [J].
KORSCHING, S ;
AUBURGER, G ;
HEUMANN, R ;
SCOTT, J ;
THOENEN, H .
EMBO JOURNAL, 1985, 4 (06) :1389-1393
[9]   NERVE GROWTH-FACTOR TREATMENT AFTER BRAIN INJURY PREVENTS NEURONAL DEATH [J].
KROMER, LF .
SCIENCE, 1987, 235 (4785) :214-216
[10]   THE NERVE GROWTH-FACTOR 35 YEARS LATER [J].
LEVIMONTALCINI, R .
SCIENCE, 1987, 237 (4819) :1154-1162