PRODUCTION OF NEISSERIA-GONORRHOEAE PILI (FIMBRIAE) IN PSEUDOMONAS-AERUGINOSA

被引:39
作者
HOYNE, PA
HAAS, R
MEYER, TF
DAVIES, JK
ELLEMAN, TC
机构
[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA
[2] MAX PLANCK INST BIOL,INFEKTIONSBIOL ABT,W-7400 TUBINGEN,GERMANY
关键词
D O I
10.1128/jb.174.22.7321-7327.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas aeruginosa K/2PfS, when transformed with an expression plasmid harboring the pilin gene (pilE1) of Neisseria gonorrhoeae MS11, was able to express and assemble gonococcal pilin monomers into surface-associated pili, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and immunoelectron microscopy. Concomitant with the expression of gonococcal pili in P. aeruginosa was the virtual loss of production of P. aeruginosa K/2PfS pili normally associated with the host cell.
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页码:7321 / 7327
页数:7
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