MOLECULAR-CLONING, NUCLEOTIDE-SEQUENCE AND EXPRESSION OF A SULFOLOBUS-SOLFATARICUS GENE ENCODING A CLASS-II FUMARASE

被引：17

作者：

COLOMBO, S ^{[1
]}

GRISA, M ^{[1
]}

TORTORA, P ^{[1
]}

VANONI, M ^{[1
]}

机构：

[1] UNIV MILAN,DIPARTIMENTO FISIOL & BIOCHIM GEN,I-20133 MILAN,ITALY

来源：

FEBS LETTERS | 1994年 / 337卷 / 01期

关键词：

FUMARATE HYDRATASE; ARCHAEBACTERIA; THERMOSTABLE ENZYME; GENE CLONING; SOLFOLOBUS SOLFATARICUS;

D O I：

10.1016/0014-5793(94)80636-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fumarase catalyzes the interconversion of L-malate and fumarate. A Sulfolobus solfataricus fumarase gene (fumC) was cloned and sequenced. Typical archaebacterial regulatory sites were identified in the region flanking the fumC open reading frame. The fumC gene encodes a protein of 438 amino acids (47,899 Da) which shows several significant similarities with class II fumarases from both eubacterial and eukariotic sources as well as with aspartases. S. solfataricus fumarase expressed in Escherichia coli retains enzymatic activity and its thermostability is comparable to that of S. solfataricus purified enzyme despite a 11 amino acid C-terminal deletion.

引用

页码：93 / 98

页数：6

共 34 条

[1] CLONING, SEQUENCING, AND MUTATIONAL ANALYSIS OF THE BRADYRHIZOBIUM-JAPONICUM-FUMC-LIKE GENE - EVIDENCE FOR THE EXISTENCE OF 2 DIFFERENT FUMARASES [J].