PREDICTION OF PH-DEPENDENT PROPERTIES OF DNA TRIPLE HELICES

被引：29

作者：

HUSLER, PL ^{[1
]}

KLUMP, HH ^{[1
]}

机构：

[1] UNIV CAPE TOWN,DEPT BIOCHEM,RONDEBOSCH 7700,SOUTH AFRICA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 317卷 / 01期

关键词：

D O I：

10.1006/abbi.1995.1134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thermodynamic properties of two triple helices were investigated by uv thermal denaturation, differential scanning calorimetry, and pH titrations. Starting from the grand partition function and using matrix methods we present a formalism that describes pH effects on the thermal stability of triple helices. The formalism can be used over a wide pH range and is not restricted to the limiting case where the pH is larger or smaller than the pK(a) of cytosine. Furthermore, it covers nearest neighbor electrostatic effects of closely spaced cytosines in the Hoogsteen strand which can shift the pK(a) of cytosine to lower pH values. A procedure is employed to predict enthalpy and entropy changes for tripler formation. These values are in accordance with the results obtained by differential scanning calorimetry. (C) 1995 Academic Press, Inc.

引用

页码：46 / 56

页数：11

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