MOLECULAR AND CELLULAR CHARACTERIZATION OF THE GABA(A) RECEPTOR IN THE RAT PANCREAS

被引：27

作者：

BORBONI, P

PORZIO, O

FUSCO, A

SESTI, G

LAURO, R

MARLIER, LNJL

机构：

[1] Department of Internal Medicine, University of Rome Tor Vergata, 00173 Rome, Via Orazio Raimondo

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1994年 / 103卷 / 1-2期

关键词：

GABA(A) RECEPTOR; GENE EXPRESSION; RT-PCR; PANCREATIC ISLET; INSULINOMA CELL LINE;

D O I：

10.1016/0303-7207(94)90083-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the present study, we characterize the molecular structure of the GABA(A) receptor in pancreas, islets, alpha and beta cells, and in RIN 1046-38 cells. Using the polymerase chain reaction and specific primers for 11 out of the 15 subunits known so far, that may contribute to the composition of the GABA(A) receptors, we demonstrate that pancreas and its cellular components, as well RIN 1046-38 cells, might contain a GABA(A) receptor resulting from all the possible combinations in a pentameric configuration of the subtypes alpha 1, alpha 2, alpha 3 of the alpha subunit family, beta 1, beta 2, beta 3 subtypes of the beta subunit family, delta subunit and gamma 2 subtype of the gamma subunit family. The presence of the gamma 2 subunit renders the GABA(A) receptors potentially sensitive to allosteric modulators.

引用

页码：157 / 163

页数：7

共 44 条

[1] IDENTIFICATION OF THE 64K AUTOANTIGEN IN INSULIN-DEPENDENT DIABETES AS THE GABA-SYNTHESIZING ENZYME GLUTAMIC-ACID DECARBOXYLASE [J].