SEPARATION OF THE GAMMA-A AND GAMMA-G CYANOGEN-BROMIDE PEPTIDES OF HUMAN-FETAL HEMOGLOBIN BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：15

作者：

STOMING, TA ^{[1
]}

GARVER, FA ^{[1
]}

GANGAROSA, MA ^{[1
]}

HARRISON, JM ^{[1
]}

HUISMAN, THJ ^{[1
]}

机构：

[1] COLL GEORGIA,DEPT MED,AUGUSTA,GA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90561-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Gγ and Aγ variants of human fetal hemoglobin are defined on the basis of a single glycine/alanine substitution at position 136 of the γ chain which is located in the carboxyterminal cyanogen bromide fragment (γCB-3). This communication describes the rapid separation of these two tridecapeptides by high-pressure liquid chromatography. Application of this new technique will facilitate the study of the biosynthesis and genetics of human fetal hemoglobin. © 1979.

引用

页码：113 / 117

页数：5

共 7 条

[1]

ABRAHAM E C, 1976, Hemoglobin, V1, P27, DOI 10.3109/03630267609031020

[2] Protein coagulation and its reversal - The preparation of insoluble globin, soluble globin and heme [J].