PARTIAL-PURIFICATION OF HUMAN PROSTATIC 5-ALPHA-REDUCTASE (3-OXO-5-ALPHA-STEROID-NADP+ 4-ENE-OXIDO-REDUCTASE, EC 1.3.1.22) IN A STABLE AND ACTIVE FORM

被引：34

作者：

SARGENT, NSE ^{[1
]}

HABIB, FK ^{[1
]}

机构：

[1] UNIV EDINBURGH,WESTERN GEN HOSP,DEPT SURG,CREWE RD S,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1991年 / 38卷 / 01期

关键词：

D O I：

10.1016/0960-0760(91)90403-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5-alpha-reductase was active and stable for at least three weeks and showed an increase in specific activity (V(max) +/- SD = 48.9 +/- 7.4 pmol DHT/mg protein/ml) over that of the starting homogenate (V(max) +/- SD = 5.6 +/- 1.5 pmol DHT/mg protein/min) of 8.7 times.

引用

页码：73 / 77

页数：5

共 15 条

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