THE 3RD MOLECULE ASSOCIATED WITH INTERLEUKIN-2 RECEPTOR-ALPHA AND BETA-CHAIN

被引：8

作者：

KAMIO, M

ARIMA, N

TSUDO, M

IMADA, K

OHKUMA, M

UCHIYAMA, T

机构：

[1] KYOTO UNIV,INST ANIM PHYSIOL,HUMAN IMMUNODEFICIENCY VIRUS RES INST,SAKYO KU,KYOTO 606,JAPAN

[2] UNITIKA CENT HOSP,DEPT INTERNAL MED,UJI,KYOTO 611,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80022-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is known that the affinity cross-linking study of the human high-affinity Interleukin 2 (IL-2) receptor reveals triplet bands consisting of 70 kDa α chain(Tac)-IL-2 and the 90/80 kDa doublet. We found the cell lines lacking the lower band of the doublet in spite of the expression of both α and β chains. No IL-2 binding was detectable in the presence of anti-Tac antibody in these cells. Immunoprecipitation from the cell extract of [125I] IL-2-cross-linked T cells with anti-β chain polyclonal IgG detected the upper band, but not lower band of the doublet. These data suggest that the lower band of the doublet represents an unknown IL-2-binding protein (p65) distinct from the β chain and this molecule may be involved in the intermediate-affinity IL-2 binding together with the β chain. © 1992 Academic Press, Inc.

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页码：1288 / 1292

页数：5

相关论文

共 18 条

[1]

ARIMA N, UNPUB

[2] INTERLEUKIN-2 RECEPTOR BETA-CHAIN GENE - GENERATION OF 3 RECEPTOR FORMS BY CLONED HUMAN ALPHA-CHAIN AND BETA-CHAIN CDNAS [J].

HATAKEYAMA, M ;

TSUDO, M ;

MINAMOTO, S ;

KONO, T ;

DOI, T ;

MIYATA, T ;

MIYASAKA, M ;

TANIGUCHI, T .

SCIENCE, 1989, 244 (4904) :551-556

[3] THE HUMAN INTERMEDIATE-AFFINITY INTERLEUKIN-2 RECEPTOR CONSISTS OF 2 DISTINCT, PARTIALLY HOMOLOGOUS GLYCOPROTEINS [J].

HERRMANN, T ;

DIAMANTSTEIN, T .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (07) :1051-1057

[4] THE HIGH-AFFINITY INTERLEUKIN-2 RECEPTOR - EVIDENCE FOR 3 DISTINCT POLYPEPTIDE-CHAINS COMPRISING THE HIGH-AFFINITY INTERLEUKIN-2 RECEPTOR [J].

HERRMANN, T ;

DIAMANTSTEIN, T .

MOLECULAR IMMUNOLOGY, 1988, 25 (11) :1201-1207

[5]

HORI T, 1988, J IMMUNOL, V140, P4199

[6] A MONOCLONAL-ANTIBODY THAT APPEARS TO RECOGNIZE THE RECEPTOR FOR HUMAN T-CELL GROWTH-FACTOR - PARTIAL CHARACTERIZATION OF THE RECEPTOR [J].

LEONARD, WJ ;

DEPPER, JM ;

UCHIYAMA, T ;

SMITH, KA ;

WALDMANN, TA ;

GREENE, WC .

NATURE, 1982, 300 (5889) :267-269

[7] INTERLEUKIN-2 BINDING MOLECULE DISTINCT FROM THE TAC PROTEIN - ANALYSIS OF ITS ROLE IN FORMATION OF HIGH-AFFINITY RECEPTORS [J].

ROBB, RJ ;

RUSK, CM ;

YODOI, J ;

GREENE, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :2002-2006

[8] A MONOCLONAL-ANTIBODY 7G7/B6, BINDS TO AN EPITOPE ON THE HUMAN INTERLEUKIN-2 (IL-2) RECEPTOR THAT IS DISTINCT FROM THAT RECOGNIZED BY IL-2 OR ANTI-TAC [J].

RUBIN, LA ;

KURMAN, CC ;

BIDDISON, WE ;

GOLDMAN, ND ;

NELSON, DL .

HYBRIDOMA, 1985, 4 (02) :91-102

[9] EVIDENCE FOR ADDITIONAL SUBUNITS ASSOCIATED TO THE MOUSE INTERLEUKIN-2 RECEPTOR P55/P75 COMPLEX [J].

SARAGOVI, H ;

MALEK, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :11-15

[10] A 100-KILODALTON PROTEIN IS ASSOCIATED WITH THE MURINE INTERLEUKIN-2 RECEPTOR - BIOCHEMICAL-EVIDENCE THAT P100 IS DISTINCT FROM THE ALPHA-CHAIN AND BETA-CHAIN [J].

SHARON, M ;

GNARRA, JR ;

LEONARD, WJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4869-4873