EVIDENCE FOR COPPER AND 3,4,6-TRIHYDROXYPHENYLALANINE QUINONE COFACTORS IN AN AMINE OXIDASE FROM THE GRAM-NEGATIVE BACTERIUM ESCHERICHIA-COLI K-12

被引：85

作者：

COOPER, RA

KNOWLES, PF

BROWN, DE

MCGUIRL, MA

DOOLEY, DM

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,ENGLAND

[2] UNIV LEEDS,DEPT BIOCHEM & MOLEC BIOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

[3] AMHERST COLL,DEPT CHEM,AMHERST,MA 01002

来源：

BIOCHEMICAL JOURNAL | 1992年 / 288卷

基金：

英国惠康基金;

关键词：

D O I：

10.1042/bj2880337

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cofactors present in an amine oxidase induced in Escherichia coli K-12 by growth on 2-phenylethylamine have been studied by spectroscopic methods. E.s.r. spectroscopy establishes the presence of cupric copper while resonance Raman spectroscopy on the phenylhydrazine derivative of the enzyme provides strong evidence for the oxidized form of 3,4,6-trihydroxyphenylalanine (TOPA) quinone. The amine oxidase should accordingly be classified as EC 1.4.3.6. This is the first report of such an amine oxidase in a Gram-negative bacterium.

引用

页码：337 / 340

页数：4

共 17 条

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[3]

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