EXPRESSION OF THE POLYHOMEOTIC LOCUS IN DEVELOPMENT OF DROSOPHILA-MELANOGASTER

被引:18
作者
DECAMILLIS, M
BROCK, HW
机构
[1] UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER V6T 1Z4,BC,CANADA
[2] KANSAS STATE UNIV AGR & APPL SCI,DIV BIOL,MANHATTAN,KS 66506
来源
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY | 1994年 / 203卷 / 7-8期
关键词
EMBRYOS; IMAGINAL DISKS; OVARIES; SEGMENTATION GENES; CHROMATIN;
D O I
10.1007/BF00188692
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The polyhomeotic (ph) gene of Drosophila melanogaster encodes a chromatin protein required for negative regulation of homeotic genes, and is a member of the Polycomb group of genes. The distribution of ph mRNA and protein was determined in embryos, imaginal discs, and ovaries. Distribution of ph protein and mRNA coincided, except in early embryogogenesis. During blastoderm, ph mRNA is present in anterior and posterior domains that are themselves subdivided into stripes. During germ band extension, a segmentally repeated striped pattern of mRNA expression is seen. ph protein is first detected as a nuclear protein during cell cycle 10, and is ubiquitously expressed. ph protein stains more heavily in the ectodermal mitotic domains de scribed by Foe (1989). Later, ph mRNA and protein expression is concentrated in the neuronal cell bodies of the central nervous system, and can also be detected in the peripheral nervous system. In imaginal discs, ph expression is non-uniform in metathoracic discs, but appears more regular in other imaginal discs. The ph mRNA is found in the germarium and in stages 1-10 in nurse cells and follicle cells, but we do not detect it in oocytes. These results are discussed with respect to the expression of Polycomb, and with respect to the function of the Polycomb group.
引用
收藏
页码:429 / 438
页数:10
相关论文
共 46 条
[1]   MATERNAL EXPRESSION OF GENES THAT REGULATE THE BITHORAX COMPLEX OF DROSOPHILA-MELANOGASTER [J].
BREEN, TR ;
DUNCAN, IM .
DEVELOPMENTAL BIOLOGY, 1986, 118 (02) :442-456
[2]  
BRUNK BP, 1991, GENETICS, V128, P119
[3]  
Bryant P.J., 1978, BIOL DROSOPH, V2c, P229
[4]  
Campos-Ortega J. A., 1985, EMBRYONIC DEV DROSOP
[5]   DETECTION OF MESSENGER-RNAS IN SEA-URCHIN EMBRYOS BY INSITU HYBRIDIZATION USING ASYMMETRIC RNA PROBES [J].
COX, KH ;
DELEON, DV ;
ANGERER, LM ;
ANGERER, RC .
DEVELOPMENTAL BIOLOGY, 1984, 101 (02) :485-502
[6]   THE COMPLEX GENETIC-LOCUS POLYHOMEOTIC IN DROSOPHILA-MELANOGASTER POTENTIALLY ENCODES 2 HOMOLOGOUS ZINC-FINGER PROTEINS [J].
DEATRICK, J ;
DALY, M ;
RANDSHOLT, NB ;
BROCK, HW .
GENE, 1991, 105 (02) :185-195
[7]   LOCALIZATION INSITU OF POLYHOMEOTIC TRANSCRIPTS IN DROSOPHILA EMBRYOS REVEALS SPATIALLY RESTRICTED EXPRESSION BEGINNING AT THE BLASTODERM STAGE [J].
DEATRICK, J .
DEVELOPMENTAL GENETICS, 1992, 13 (05) :326-330
[8]   THE POLYHOMEOTIC GENE OF DROSOPHILA ENCODES A CHROMATIN PROTEIN THAT SHARES POLYTENE CHROMOSOME-BINDING SITES WITH POLYCOMB [J].
DECAMILLIS, M ;
CHENG, NS ;
PIERRE, D ;
BROCK, HW .
GENES & DEVELOPMENT, 1992, 6 (02) :223-232
[9]  
DUNCAN I, 1982, DEV ORDER ITS ORIGIN, V40, P553
[10]   MATERNAL AND ZYGOTIC REQUIREMENT FOR THE POLYHOMEOTIC COMPLEX GENETIC-LOCUS IN DROSOPHILA [J].
DURA, JM ;
DEATRICK, J ;
RANDSHOLT, NB ;
BROCK, HW ;
SANTAMARIA, P .
ROUXS ARCHIVES OF DEVELOPMENTAL BIOLOGY, 1988, 197 (04) :239-246