STUDIES ON CELL-WALL POLYSACCHARIDES FROM SIPHONOUS GREEN-ALGAE .4. STUDIES ON THE CELL-WALL MANNAN OF THE SIPHONOUS GREEN-ALGAE, CODIUM-LATUM

被引：13

作者：

KAIHOU, S ^{[1
]}

HAYASHI, T ^{[1
]}

OTSURU, O ^{[1
]}

MAEDA, M ^{[1
]}

机构：

[1] SAITAMA UNIV,DEPT BIOCHEM,URAWA,SAITAMA 338,JAPAN

来源：

CARBOHYDRATE RESEARCH | 1993年 / 240卷

关键词：

D O I：

10.1016/0008-6215(93)84184-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-wall mannan from Codium latum was completely solubilized as methylol mannan from their microfibrils by treatment with paraformaldehyde-Me2SO systems at 150-degrees-C, and it was purified by gel-permeation chromatography with Toyopearl gel under Me2SO elution. Mannan was regenerated by dilution of the purified methylol mannan with water or methanol. The elution profile of regenerated mannan on gel permeation chromatography under Me2SO or the IR spectrum of the deuterium-exchanged, fractionated mannan revealed that the cell-wall mannan was aggregated as higher molecular weight material through intermolecular hydrogen bonding to form a difficultly soluble material. The results of structural determination on the purified mannan, including periodate oxidation and permethylation analysis, revealed a (1 --> 4)-beta-linked linear polysaccharide, as well as other cell-wall microfibril polysaccharides.

引用

页码：207 / 218

页数：12

共 23 条

[1] A NEW PROCEDURE FOR THE DETERMINATION OF THE FINE STRUCTURE OF POLYSACCHARIDES [J].