ACTIN FILAMENT DYNAMICS IN LIVING GLIAL-CELLS IMAGED BY ATOMIC FORCE MICROSCOPY

被引：369

作者：

HENDERSON, E

HAYDON, PG

SAKAGUCHI, DS

机构：

[1] Department of Zoology and Genetics, Signal Transduction Training Group, Iowa State University, Ames

来源：

SCIENCE | 1992年 / 257卷 / 5078期

关键词：

D O I：

10.1126/science.1411511

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Observation of filamentous actin (F-actin) in living cells is currently limited to the resolution of the light microscope. Higher resolution procedures require sample fixation and preclude dynamic studies. The atomic force microscope (AFM) can image and manipulate samples at very high, sometimes atomic resolution by scanning a fine tip over the surface of interest and detecting physical interactions between the tip and sample. This study demonstrates that F-actin can be readily resolved in living cells with the AFM and that the dynamic properties of F-actin are easily observed.

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页码：1944 / 1946

页数：3

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