NUCLEOTIDE-SEQUENCE OF A FLOWER-SPECIFIC MADS BOX CDNA CLONE FROM ORCHID

被引:67
作者
LU, ZX [1 ]
WU, M [1 ]
LOH, CS [1 ]
YEONG, CY [1 ]
GOH, CJ [1 ]
机构
[1] NATL UNIV SINGAPORE,DEPT BOT,LOWER KENT RIDGE RD,SINGAPORE 0511,SINGAPORE
关键词
ORCHID; ARANDA-DEBORAH; MONOCOTYLEDON; FLORAL-SPECIFIC GENE; MADS BOX;
D O I
10.1007/BF00021545
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An orchid (Aranda deborah) mature flower cDNA library was screened with an agamous cDNA probe from Arabidopsis. One positive clone for agamous gene was isolated, cloned and sequenced. This cDNA clone (om1) has a full length open reading frame of 750 bp corresponding to 250 amino acid residues. Comparison of om1 MADS box with that of its counterparts in tomato and Arabidopisis reveals significantly high homology (> 95 %). Northern analysis indicated this gene is expressed in mature flowers and not in young developing inflorescences or young floral buds. In the mature flowers, it is only expressed in petals and weakly in sepals but not in the column (gynostemium).
引用
收藏
页码:901 / 904
页数:4
相关论文
共 12 条