IDENTIFICATION AND TIME OF SYNTHESIS OF CHORION PROTEINS IN DROSOPHILA-MELANOGASTER

被引:115
作者
WARING, GL [1 ]
MAHOWALD, AP [1 ]
机构
[1] INDIANA UNIV, DEPT BIOL, PROGRAM MOLEC CELLULAR & DEV BIOL, BLOOMINGTON, IN 47401 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1016/0092-8674(79)90033-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The chorion of Drosophila melanogaster consists of proteins secreted by the follicular epithelium during late oogenesis. Petri, Wyman and Kafatos (1976) have described six major protein components of the Drosophila chorion and reported the synthesis of these proteins in vitro by mass-isolated egg chambers. We have used two-dimensional gel electrophoresis to identify approximately twenty components in highly purified chorion preparations. The synthesis patterns of these proteins in vivo were determined by isolating egg chambers of different developmental stages from flies injected with 14C amino acids. Chorion proteins constitute a large fraction of the protein synthesized by ovarian egg chambers in stages 12-14. The sizes and times of synthesis of the chorion proteins correlate closely with the production of poly(A)-containing RNAs by the follicle cells (Spradling and Mahowald, 1979). © 1979.
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页码:599 / 607
页数:9
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