CHLOROPHYLL-B INTERFERENCE IN THE FLUOROMETRIC-DETERMINATION OF CHLOROPHYLL-A AND PHAEO-PIGMENTS

被引：54

作者：

GIBBS, CF

机构：

[1] Marine Studies Group, Ministry for Conservation, East Melbourne, VIC, 3002

来源：

AUSTRALIAN JOURNAL OF MARINE AND FRESHWATER RESEARCH | 1979年 / 30卷 / 05期

关键词：

D O I：

10.1071/MF9790597

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A quantitative estimate was made of the interference by chlorophyll b in the fluorometric determination of chlorophyll a using an Aminco-Bowman spectrofiuorometer. The undegraded (or ‘living’) chlorophyll a concentration was underestimated by an amount equal to the concentration of chlorophyll b present. The value for chlorophyll a uncorrected for phaeophytin was increased by 30% of the chlorophyll b concentration. The acetone to water ratio was found to affect the acidification factor only at aqueous acetone concentrations above 90%. The need for standardization of solvent and wavelengths is discussed, and the implications of chlorophyll b occurring in marine samples are considered. © 1979 ASEG.

引用

页码：597 / 606

页数：10

共 14 条

[1]

BARRETT J, 1971, Journal of Experimental Marine Biology and Ecology, V7, P255, DOI 10.1016/0022-0981(71)90008-6

[2] SELECTIVE EXCITATION FLUOROMETRY FOR DETERMINATION OF CHLOROPHYLLS AND PHEOPHYTINS [J].