HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TYLOSIN IN BOVINE MUSCLE, KIDNEY AND LIVER

A method is described for the determination of the residual macrolide antibiotic, tylosin, in bovine muscle kidney, and liver by high performance liquid chromatography. Tylosin was extracted from the tissues with diochloromethane, concentrated on a silica gel solid phase extraction cartridge, and analysed using a reversed-phase C18 column, with UV detection at 280 nm and a mobile phase of 0.005 M ammonium dihydrogen phosphate-acetonitrile-methanol (8:72:20) (v/v/v). The method, which has a detection limit of 15 ng/g in kidney, liver, and muscle, vas validated by demonstrating that it is accurate, precise, and reproducible and provides high recovery from tissues fortified with tylosin.