CHARACTERIZATION OF A BIS(5'-NUCLEOSIDYL) TRIPHOSPHATE PYROPHOSPHOHYDROLASE FROM ENCYSTED EMBRYOS OF THE BRINE SHRIMP ARTEMIA

被引：14

作者：

PRESCOTT, M ^{[1
]}

THORNE, NMH ^{[1
]}

MILNE, AD ^{[1
]}

MCLENNAN, AG ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT BIOCHEM,POB 147,LIVERPOOL L69 3BX,ENGLAND

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1992年 / 24卷 / 04期

关键词：

D O I：

10.1016/0020-711X(92)90328-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A P1,P3-his(5'-nucleosidyl)triphosphate pyrophosphohydrolase (Np3Nase) has been partially purified from Artemia embryos. 2. The Np3 Nase has a native M(r) of 115,000 and preferentially hydrolyses substrates of the form Np3N. Relative rates of hydrolysis are Ap3A (V(rel) = 1.0), Gp3G (V(rel) = 0.71), Ap4A (V(rel) = 0.08), Ap5A (V(rel) = 0.09), Gp4G (V(rel) = 0.3) and Gp5G (V(rel) = 0.33). An NMP is always one of the products. 3. The K(m) values for Ap3A and Gp3G are 15 and 10-mu-M respectively. 4. Mg2+, Mn2+ and Ca2+ ions all stimulate the activity, while Zn2+, Co2+ and Ni2+ ions are inhibitory. 5. The activity of the NP3Nase remains constant during pre-emergence development of encysted embryos but decreases slightly after hatching.

引用

页码：565 / 571

页数：7

共 32 条

[1] ALTERATIONS IN LEVELS OF 5'-ADENYL DINUCLEOTIDES FOLLOWING DNA DAMAGE IN NORMAL HUMAN-FIBROBLASTS AND FIBROBLASTS DERIVED FROM PATIENTS WITH XERODERMA PIGMENTOSUM [J].

BAKER, JC ;

AMES, BN .

MUTATION RESEARCH, 1988, 208 (02) :87-93

[2] ALTERATION OF ADENYL DINUCLEOTIDE METABOLISM BY ENVIRONMENTAL-STRESS [J].

BAKER, JC ;

JACOBSON, MK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2350-2352

[3]

BLANQUET S, 1983, MOL CELL BIOCHEM, V52, P3

[4] APPPPA AND RELATED ADENYLYLATED NUCLEOTIDES ARE SYNTHESIZED AS A CONSEQUENCE OF OXIDATION STRESS [J].

BOCHNER, BR ;

LEE, PC ;

WILSON, SW ;

CUTLER, CW ;

AMES, BN .

CELL, 1984, 37 (01) :225-232

[5] OCCURRENCE OF DINUCLEOSIDETRIPHOSPHATASE IN THE CYTOSOL AND PARTICULATE FRACTIONS FROM RAT-LIVER [J].

COSTAS, MJ ;

CAMESELLE, JC ;

SILLERO, MAG ;

SILLERO, A .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1985, 17 (08) :903-909

[6] DINUCLEOSIDETRIPHOSPHATASE FROM RAT-BRAIN [J].

COSTAS, MJ ;

MONTERO, JM ;

CAMESELLE, JC ;

SILLERO, MAG ;

SILLERO, A .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1984, 16 (07) :757-762

[7]

COSTAS MJ, 1986, J BIOL CHEM, V261, P2064

[8] PARTICULATE DIADENOSINE 5',5'''-P1,P3-TRIPHOSPHATE HYDROLASES IN RAT-BRAIN - 2 SPECIFIC DINUCLEOSIDE TRIPHOSPHATASES AND 2 PHOSPHODIESTERASE I-LIKE HYDROLASES [J].

GARCIAAGUNDEZ, JA ;

CAMESELLE, JC ;

COSTAS, MJ ;

SILLERO, MAG ;

SILLERO, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1073 (02) :402-409

[9] INVIVO LEVELS OF DIADENOSINE TETRAPHOSPHATE AND ADENOSINE TETRAPHOSPHO-GUANOSINE IN PHYSARUM-POLYCEPHALUM DURING THE CELL-CYCLE AND OXIDATIVE STRESS [J].

GARRISON, PN ;

MATHIS, SA ;

BARNES, LD .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (04) :1179-1186

[10] DIADENOSINE 5',5'''-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE FROM PHYSARUM-POLYCEPHALUM - SUBSTRATE-SPECIFICITY [J].

GARRISON, PN ;

ROBERSON, GM ;

CULVER, CA ;

BARNES, LD .

BIOCHEMISTRY, 1982, 21 (24) :6129-6133

← 1 2 3 4 →