AGROBACTERIUM-MEDIATED TRANSFORMATION OF CARNATION (DIANTHUS-CARYOPHYLLUS L)

被引：60

作者：

LU, CY

NUGENT, G

WARDLEYRICHARDSON, T

CHANDLER, SF

YOUNG, R

DALLING, MJ

机构：

[1] Calgcnc Pacific Pty Ltd, Collingwood, VIC, 3066

[2] Division of Plant Industry, CSIRO, Canberra, ACT, 2601

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 09期

关键词：

D O I：

10.1038/nbt0991-864

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have recovered transgenic carnation plants on kanamycin-containing medium after co-cultivation of stems with disarmed or armed strains of Agrobacterium tumefaciens. Both strains contained a vector carrying the neomycin phosphotransferase II (NPTII) and beta-glucuronidase (GUS) genes. Optimal conditions for transformation and patterns of GUS expression in transgenic plants are described. Over two hundred transgenic plants have been established in soil.

引用

页码：864 / 868

页数：5

共 32 条

[1] THE CAMV S-35 ENHANCER CONTAINS AT LEAST 2 DOMAINS WHICH CAN CONFER DIFFERENT DEVELOPMENTAL AND TISSUE-SPECIFIC EXPRESSION PATTERNS [J].