CA2+ IS AN OBLIGATORY INTERMEDIATE IN THE EXCITATION CASCADE OF LIMULUS PHOTORECEPTORS

被引：66

作者：

SHIN, JH ^{[1
]}

RICHARD, EA ^{[1
]}

LISMAN, JE ^{[1
]}

机构：

[1] BRANDEIS UNIV, CTR COMPLEX SYST, WALTHAM, MA 02254 USA

来源：

NEURON | 1993年 / 11卷 / 05期

关键词：

D O I：

10.1016/0896-6273(93)90114-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have investigated the role of Ca2+ in the excitation of Limulus photoreceptors by intracellular injection of the Ca2+ buffer, 5,5'-dibromo-BAPTA. Buffer with free Ca2+ of 0.5 or 5 muM slowed the rising edge of the light response over 100-fold and greatly reduced both the transient and plateau phases of the light response, as expected if Ca2+ elevation is necessary for all phases of excitation. Injection of buffers with free Ca2+ of 5 or 45 muM, levels normally reached during light, evoked sustained inward current as expected if Ca2+ is sufficient for excitation. The transduction cascade appears due to a single pathway that sequentially involves 1,4,5-trisphosphate inositol, Ca2+, and cyclic GMP.

引用

收藏

页码：845 / 855

页数：11

相关论文

共 56 条

[1]

ADLER EM, 1991, J NEUROSCI, V11, P1496

[2]

BACIGALUPO J, 1990, SER CENT ES, P27

[3] LIGHT-DEPENDENT CHANNELS FROM EXCISED PATCHES OF LIMULUS VENTRAL PHOTORECEPTORS ARE OPENED BY CGMP [J].

BACIGALUPO, J ;

JOHNSON, EC ;

VERGARA, C ;

LISMAN, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7938-7942

[4] LOCALIZATION OF PHOTOTRANSDUCTION IN LIMULUS VENTRAL PHOTORECEPTORS - A DEMONSTRATION USING CELL-FREE RHABDOMERIC VESICLES [J].

BACIGALUPO, J ;

JOHNSON, EC .

VISUAL NEUROSCIENCE, 1992, 8 (01) :41-47

[5]

BAER KM, 1988, J BIOL CHEM, V263, P17

[6] CALCIUM-SENSITIVITY AND INOSITOL POLYPHOSPHATE-SENSITIVITY OF THE CALCIUM-SEQUESTERING ENDOPLASMIC-RETICULUM IN THE PHOTORECEPTOR CELLS OF THE HONEYBEE DRONE [J].

BAUMANN, O ;

WALZ, B .

JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1989, 165 (05) :627-636

[7] BELL-SHAPED CALCIUM-RESPONSE CURVES OF INS(1,4,5)P3-GATED AND CALCIUM-GATED CHANNELS FROM ENDOPLASMIC-RETICULUM OF CEREBELLUM [J].

BEZPROZVANNY, I ;

WATRAS, J ;

EHRLICH, BE .

NATURE, 1991, 351 (6329) :751-754

[8] ISOLATION OF A PUTATIVE PHOSPHOLIPASE-C GENE OF DROSOPHILA, NORPA, AND ITS ROLE IN PHOTOTRANSDUCTION [J].

BLOOMQUIST, BT ;

SHORTRIDGE, RD ;

SCHNEUWLY, S ;

PERDEW, M ;

MONTELL, C ;

STELLER, H ;

RUBIN, G ;

PAK, WL .

CELL, 1988, 54 (05) :723-733

[9] LIGHT-ACTIVATED GUANOSINE-TRIPHOSPHATASE IN MUSCA EYE MEMBRANES RESEMBLES THE PROLONGED DEPOLARIZING AFTERPOTENTIAL IN PHOTORECEPTOR CELLS [J].

BLUMENFELD, A ;

ERUSALIMSKY, J ;

HEICHAL, O ;

SELINGER, Z ;

MINKE, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (20) :7116-7120

[10] CALCIUM-ION, AN INTRACELLULAR MESSENGER OF LIGHT ADAPTATION, ALSO PARTICIPATES IN EXCITATION OF LIMULUS PHOTORECEPTORS [J].

BOLSOVER, SR ;

BROWN, JE .

JOURNAL OF PHYSIOLOGY-LONDON, 1985, 364 (JUL) :381-393

← 1 2 3 4 5 6 →