THE CU(A) SITE OF THE CAA(3)-TYPE OXIDASE OF BACILLUS-SUBTILIS IS A MIXED-VALENCE BINUCLEAR COPPER CENTER

被引：100

作者：

VONWACHENFELDT, C

DEVRIES, S

VANDEROOST, J

机构：

[1] FREE UNIV AMSTERDAM,BIOCENTRUM,DEPT MOLEC & CELLULAR BIOL,1081 HV AMSTERDAM,NETHERLANDS

[2] TECH UNIV DELFT,DEPT MICROBIOL & ENZYMOL,2628 BC DELFT,NETHERLANDS

来源：

FEBS LETTERS | 1994年 / 340卷 / 1-2期

关键词：

CYTOCHROME C OXIDASE; CU(A); BINUCLEAR COPPER CENTER; NITROUS OXIDE REDUCTASE;

D O I：

10.1016/0014-5793(94)80182-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A copper-containing domain of the caa(3)-type oxidase from Bacillus subtilis has been expressed as a water-soluble protein in the cytoplasm of Escherichia coli. Electron paramagnetic resonance (EPR) spectra of this purple domain show well-resolved lines in the g(z) resonance, both at X-band and S-band frequencies. Interpretation of EPR spectra and analytical data indicate a binuclear copper site consisting of one Cu2+ and one Cu1+. This copper site closely resembles Cu-A in subunit II of cytochrome c oxidase and is shown here to be a mixed-valence [Cu2+-Cu1+] binuclear centre.

引用

页码：109 / 113

页数：5

共 23 条

[1] A COMPARATIVE EPR INVESTIGATION OF THE MULTICOPPER PROTEINS NITROUS-OXIDE REDUCTASE AND CYTOCHROME-C-OXIDASE [J].