NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF PEANUT STRIPE POTYVIRUS

被引：28

作者：

GUNASINGHE, UB ^{[1
]}

FLASINSKI, S ^{[1
]}

NELSON, RS ^{[1
]}

CASSIDY, BG ^{[1
]}

机构：

[1] SAMUEL ROBERTS NOBLE FDN INC,DIV PLANT BIOL,ARDMORE,OK 73402

来源：

JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷

关键词：

D O I：

10.1099/0022-1317-75-9-2519

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A blotch isolate of peanut stripe virus (PStV) was cloned, sequenced and compared with other full-length potyvirus sequences. The viral genome was 10059 nucleotides (nt) in length excluding the poly(A) tail. Two potential AUG start codons were identified in the 5' non-translated region. Analysis of in vitro translation products from transcripts containing the first 600 nt of the PStV genome indicated that the first AUG (nt 134 to 136) was preferred over the second AUG (nt 146 to 148) for initiation of translation. Within the single large open reading frame, eight processed proteins were predicted. In general, motifs conserved in other potyviral sequences were also found in the PStV genome. The presence of a 6K protein between the P3 and CI proteins was predicted. An altered amino acid motif, from FI(V)VRG to FMIIRG, within the carboxyl terminus of the P1 protein, separates the PStV sequence from the majority of potyvirus sequences. Based on comparisons with available full-length potyvirus genome sequences, PStV was found to be most closely related to soybean mosaic virus.

引用

页码：2519 / 2525

页数：7

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