ISOLATION OF DNA FROM BLOOD

被引：46

作者：

KENDALL, TL ^{[1
]}

BYERLEY, DJ ^{[1
]}

DEAN, R ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT ANIM & DAIRY SCI,ATHENS,GA 30602

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90297-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation. This isolation method readily adapts to multiple samples. The DNA is characterized by high yield, solubility, lack of protein contamination, and ease of restriction endonuclease digestion. © 1991.

引用

页码：74 / 76

页数：3

共 13 条

[1] RAPID ISOLATION OF EUKARYOTIC DNA [J].