ISOLATION OF YEAST HISTONE GENES H2A AND H2B

被引：242

作者：

HEREFORD, L

FAHRNER, K

WOOLFORD, J

ROSBASH, M

KABACK, DB

机构：

[1] BRANDEIS UNIV,ROSENSTIEL BAS MED SCI RES CTR,WALTHAM,MA 02154

[2] CALTECH,DIV CHEM,PASADENA,CA 91125

来源：

CELL | 1979年 / 18卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(79)90237-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of cloned sequences for yeast histone genes H2A and H2B reveals that there are only two copies of this pair of genes within the haploid yeast genome. Within each copy, the genes for H2A and H2B are separated by approximately 700 bp of spacer DNA. The two copies are separated from one another in the yeast genome by a minimum distance of 35-60 kb. Sequence homology between the two copies is restricted to the genes for H2A and H2B; the spacer DNA between the genes is nonhomologous. In both copies, the genes for H2A and H2B are divergently transcribed. In addition, both plasmids code for other nonhistone proteins. Sequences coding for histories H3 and H4 have not been detected in the immediate vicinity of the genes for H2A and H2B. © 1979.

引用

页码：1261 / 1271

页数：11

共 30 条

[1] STRUCTURE OF M6, A RECOMBINANT PLASMID CONTAINING DICTYOSTELIUM DNA HOMOLOGOUS TO ACTIN MESSENGER-RNA [J].