DETECTION OF STREPTOMYCIN-RESISTANT PSEUDOMONAS-SYRINGAE PV PAPULANS IN MICHIGAN APPLE ORCHARDS

被引：20

作者：

JONES, AL

NORELLI, JL

EHRET, GR

机构：

[1] MICHIGAN STATE UNIV,PESTICIDE RES CTR,E LANSING,MI 48824

[2] CORNELL UNIV,NEW YORK STATE AGR EXPT STN,DEPT PLANT PATHOL,GENEVA,NY 14456

[3] MICHIGAN STATE UNIV,DEPT BOT & PLANT PATHOL,E LANSING,MI 48824

来源：

PLANT DISEASE | 1991年 / 75卷 / 05期

关键词：

D O I：

10.1094/PD-75-0529

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Streptomycin-resistant Pseudomonas syringae pv. papulans was recovered from blister spot lesions on fruit of the cultivar Mutsu collected from 12 of 17 apple (Malus domestica) orchards in Michigan in 1989. Resistant, but not sensitive, bacteria grew on King's medium B amended with 100-mu-g of streptomycin.ml-1. A 500-base pair DNA probe (SMP3) that is specific for the streptomycin-resistance gene in strain Psp36 of P. s. papulans hybridized with 238 of 275 streptomycin-resistant, but none of 122 streptomycin-sensitive, strains of P. s. papulans from Michigan orchards. Hybridization occurred with plasmids of several different sizes and not solely with the 108-kb plasmid associated with streptomycin resistance in New York State. DNA from resistant strains isolated from one orchard did not hybridize with the probe, indicating that these strains contain a resistance system unrelated to that in strain Psp36.

引用

页码：529 / 531

页数：3

共 10 条

[1]

BEDFORD K E, 1984, Canadian Journal of Plant Pathology, V6, P17

[2] STREPTOMYCIN RESISTANCE OF PSEUDOMONAS-SYRINGAE PV PAPULANS IN APPLE ORCHARDS AND ITS ASSOCIATION WITH A CONJUGATIVE PLASMID [J].