ATP-DEPENDENT LEUKOTRIENE EXPORT FROM MASTOCYTOMA-CELLS

被引:61
作者
SCHAUB, T [1 ]
ISHIKAWA, T [1 ]
KEPPLER, D [1 ]
机构
[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,DIV TUMOR BIOCHEM,W-6900 HEIDELBERG,GERMANY
关键词
ATP-DEPENDENT TRANSPORT; MASTOCYTOMA CELLS; LEUKOTRIENE EXPORT;
D O I
10.1016/0014-5793(91)80256-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The biosynthesis of leukotrienes (LT) C4 and B4 is followed by an export of these mediators into the extracellular space. This transport was characterized using plasma membrane vesicles prepared from mastocytoma cells and identified as an ATP-dependent primary active process. The apparent K(m)-values were 110 nM for LTC4 and 48-mu-M for ATP. The transport rate was highest for LTC4, whereas LTD4, LTE4, and N-acetyl-LTE4 were transported with relative rates of 31, 12 and 8%, respectively, at a concentration of 10 nM. LTB4 transport was also dependent on ATP. LTC4 transport was inhibited by LTD4 receptor antagonists (IC50 = 1.0-mu-M for MK-571 and 1.3-mu-M for LY245769) and by the inhibitor of leukotriene biosynthesis MK-886 (IC50 = 1.8-mu-M). The ATP-dependent export carrier for leukotrienes in leukotriene-synthesizing cells represents a novel member of the family of ATP-dependent exit pumps.
引用
收藏
页码:83 / 86
页数:4
相关论文
共 22 条
[1]   BIOLOGICALLY-ACTIVE PRODUCTS OF STIMULATED LIVER MACROPHAGES (KUPFFER CELLS) [J].
DECKER, K .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 192 (02) :245-261
[2]   LEUKOTRIENES AS MEDIATORS IN TISSUE TRAUMA [J].
DENZLINGER, C ;
RAPP, S ;
HAGMANN, W ;
KEPPLER, D .
SCIENCE, 1985, 230 (4723) :330-332
[3]   L-663,536 (MK-886) (3-[1-(4-CHLOROBENZYL)-3-T-BUTYL-THIO-5-ISOPROPYLINDOL-2-YL]-2,2-DIMETHYLPROPANOIC ACID), A NOVEL, ORALLY ACTIVE LEUKOTRIENE BIOSYNTHESIS INHIBITOR [J].
GILLARD, J ;
FORDHUTCHINSON, AW ;
CHAN, C ;
CHARLESON, S ;
DENIS, D ;
FOSTER, A ;
FORTIN, R ;
LEGER, S ;
MCFARLANE, CS ;
MORTON, H ;
PIECHUTA, H ;
RIENDEAU, D ;
ROUZER, CA ;
ROKACH, J ;
YOUNG, R ;
MACINTYRE, DE ;
PETERSON, L ;
BACH, T ;
EIERMANN, G ;
HOPPLE, S ;
HUMES, J ;
HUPE, L ;
LUELL, S ;
METZGER, J ;
MEURER, R ;
MILLER, DK ;
OPAS, E ;
PACHOLOK, S .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1989, 67 (05) :456-464
[4]   PREVENTION OF ENDOGENOUS LEUKOTRIENE PRODUCTION DURING ANAPHYLAXIS IN THE GUINEA-PIG BY AN INHIBITOR OF LEUKOTRIENE BIOSYNTHESIS (MK-886) BUT NOT BY DEXAMETHASONE [J].
GUHLMANN, A ;
KEPPLER, A ;
KASTNER, S ;
KRIETER, H ;
BRUCKNER, UB ;
MESSMER, K ;
KEPPLER, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 170 (06) :1905-1918
[5]   IDENTIFICATION OF THE MAJOR ENDOGENOUS LEUKOTRIENE METABOLITE IN THE BILE OF RATS AS N-ACETYL LEUKOTRIENE E-4 [J].
HAGMANN, W ;
DENZLINGER, C ;
RAPP, S ;
WECKBECKER, G ;
KEPPLER, D .
PROSTAGLANDINS & OTHER LIPID MEDIATORS, 1986, 31 (02) :239-251
[6]  
HULTNER L, 1989, IMMUNOLOGY, V67, P408
[7]   STRUCTURAL MODEL OF ATP-BINDING PROTEINS ASSOCIATED WITH CYSTIC-FIBROSIS, MULTIDRUG RESISTANCE AND BACTERIAL TRANSPORT [J].
HYDE, SC ;
EMSLEY, P ;
HARTSHORN, MJ ;
MIMMACK, MM ;
GILEADI, U ;
PEARCE, SR ;
GALLAGHER, MP ;
GILL, DR ;
HUBBARD, RE ;
HIGGINS, CF .
NATURE, 1990, 346 (6282) :362-365
[9]  
ISHIKAWA T, 1990, J BIOL CHEM, V265, P19279
[10]   EVIDENCE FOR LEUKOTRIENE-C4 TRANSPORT MEDIATED BY AN ATP-DEPENDENT GLUTATHIONE S-CONJUGATE CARRIER IN RAT-HEART AND LIVER PLASMA-MEMBRANES [J].
ISHIKAWA, T ;
KOBAYASHI, K ;
SOGAME, Y ;
HAYASHI, K .
FEBS LETTERS, 1989, 259 (01) :95-98