SHORT-CHAIN AND MEDIUM-CHAIN ALIPHATIC-ESTER SYNTHESIS IN SACCHAROMYCES-CEREVISIAE

被引:111
作者
MALCORPS, P [1 ]
DUFOUR, JP [1 ]
机构
[1] UNIV CATHOLIQUE LOUVAIN,UNITE BRASSERIE & IND ALIMENTAIRES,PL CROIX SUD 2-BTE 7,B-1348 LOUVAIN,BELGIUM
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 210卷 / 03期
关键词
D O I
10.1111/j.1432-1033.1992.tb17507.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the yeast Saccharomyces cerevisiae, the enzymes which catalyse the synthesis of ethyl acetate, ethyl n-hexanoate and isoamyl acetate were partly resolved from a fraction containing slowly sedimenting lipoproteins released during cell disruption with glass beads. Solubilization with detergents and fractionation by affinity chromatography have demonstrated the presence of at least three, and probably four, ester synthases which differ in their catalytic properties. Isoamyl-acetate synthase was solubilized and extensively purified to apparent homogeneity by successive chromatographies on various columns. On the basis of its specific activity in cell-free extracts, the enzyme was purified 19000-fold with a 5% activity yield. As judged by SDS/PAGE, it consists of a single polypeptide chain with a molecular mass of 57 +/- 3 kDa and its apparent pI is 5.5. The enzyme acetylates isoamyl alcohol, ethanol and 12-DL-hydroxystearic acid from acetyl-CoA but is unable to use n-hexanoyl-CoA as a cosubstrate. This enzyme, defined as an acetyl-CoA: O-alcohol acetyltransferase, could be the product of one of the anaerobically induced genes in S. cerevisiae.
引用
收藏
页码:1015 / 1022
页数:8
相关论文
共 28 条
[1]   PURIFICATION AND SOME PROPERTIES OF ALCOHOL ACETYLTRANSFERASE FROM SAKE YEAST [J].
AKITA, O ;
SUZUKI, S ;
OBATA, T ;
HARA, S .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (06) :1485-1490
[2]  
ARMSTRONG DW, 1986, ACS SYM SER, V317, P255
[3]  
AYKANEN L, 1983, AROMA BEER WINE DIST
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
Dufour J.P., 1987, P C EUR BREW CONV, V21, P377
[6]  
HAMADA M, 1985, PLANT CELL PHYSL, V26, P1067
[7]  
HOON SK, 1986, KOREAN J APPL MICROB, V14, P69
[8]  
HOSAKA K, 1984, BIOCHIM BIOPHYS ACTA, V348, P179
[9]  
Ishikawa T., 1984, Journal of the Brewing Society of Japan, V79, P62
[10]  
KURIYAMA K, 1989, Hakkokogaku Kaishi, V67, P105