REPLICATIVE INTERMEDIATES OF HUMAN PAPILLOMAVIRUS TYPE-11 IN LARYNGEAL PAPILLOMAS - SITE OF REPLICATION INITIATION AND DIRECTION OF REPLICATION

被引：21

作者：

AUBORN, KJ ^{[1
]}

LITTLE, RD ^{[1
]}

PLATT, THK ^{[1
]}

VACCARIELLO, MA ^{[1
]}

SCHILDKRAUT, CL ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT CELL BIOL, BRONX, NY 10461 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 15期

关键词：

D O I：

10.1073/pnas.91.15.7340

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have examined the structures of replication intermediates from the human papillomavirus type 11 genome in DNA extracted from papilloma lesions (laryngeal papillomas). The sites of replication initiation and termination utilized in vivo were mapped by using neutral/neutral and neutral/alkaline two-dimensional agarose gel electrophoresis methods. Initiation of replication was detected in or very close to the upstream regulatory region (URR; the noncoding, regulatory sequences upstream of the open reading frames in the papillomavirus genome). We also show that replication forks proceed bidirectionally from the origin and converge 180 degrees opposite the URR. These results demonstrate the feasibility of analysis of replication of viral genomes directly from infected tissue.

引用

页码：7340 / 7344

页数：5

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