MORTALITY OF 3RD-INSTAR CARIBBEAN FRUIT-FLY (DIPTERA, TEPHRITIDAE) REARED AT 3 TEMPERATURES AND EXPOSED TO HOT-WATER IMMERSION OR COLD-STORAGE

Caribbean fruit flies, Anastrepha suspensa (Loew), were reared from egg to third instar at 20, 25, or 30 + 0.3-degrees-C in a semiartificial diet, carambolag, and guavas, then exposed to hot water immersion (43-46-degrees-C) and cold storage (1-degrees-C) quarantine treatments. Caribbean fruit flies were more tolerant of heat when reared at higher temperatures than low temperatures inside the range of 20-30-degrees-C. Third instars reared on semiartificial diet at 30-degrees-C had significantly less mortality (49.0%) than those reared at 20-degrees-C (94.2%) when insects reared at both temperatures were immersed in water at 43-degrees-C for 21 min. In tests with large numbers of third instars reared on semiartificial diet, mortality was 100, 99.9909, and 99.926% for larvae reared at 20, 25, and 30-degrees-C, respectively, and immersed in water at 43-degrees-C for 43 min. Mortality of Caribbean fruit fly larvae reared inside guavas at 20, 25, and 30-degrees-C immersed in hot water at 46-degrees-C for 25 min was 100, 99.917, and 99.40%, respectively. This information should be considered when heat quarantine treatments and models of Caribbean fruit fly mortality to heat are designed. Rearing temperature (20-30-degrees-C) did not affect tolerance to 1-degrees-C cold storage.