EXPRESSION OF A HUMAN SERUM-ALBUMIN VARIANT WITH HIGH-AFFINITY FOR THYROXINE

被引：24

作者：

PETERSEN, CE ^{[1
]}

HA, CE ^{[1
]}

MANDEL, M ^{[1
]}

BHAGAVAN, NV ^{[1
]}

机构：

[1] UNIV HAWAII,JOHN A BURNS SCH MED,DEPT BIOCHEM & BIOPHYS,HONOLULU,HI 96822

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 214卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2402

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study a protein expression system was used to synthesize recombinant human serum albumin containing a mutation that has been shown to result in familial dysalbuminemic hyperthyroxinemia, Equilibrium dialysis was used to measure the binding of this recombinant human serum albumin with thyroxine, The association constant determined for the binding of this human serum albumin variant with thyroxine was shown to be 65-fold greater than that of recombinant normal human serum albumin. (C) 1995 Academic Press, Inc.

引用

页码：1121 / 1129

页数：9

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