METHODS AND REAGENTS - LONG AND ACCURATE PCR

被引：4

作者：

HENGEN, PN

机构：

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1994年 / 19卷 / 08期

关键词：

D O I：

10.1016/0968-0004(94)90074-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses some problems encountered during the amplification of large DNA segments by the polymerase chain reaction (PCR), acid gives tips for doing long and accurate PCR. For details on how to partake in the newsgroup, see the accompanying box.

引用

页码：341 / 341

页数：1

共 2 条

[1] PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH-FIDELITY AND HIGH-YIELD FROM LAMBDA-BACTERIOPHAGE TEMPLATES [J].

BARNES, WM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2216-2220

[2] EFFECTIVE AMPLIFICATION OF LONG TARGETS FROM CLONED INSERTS AND HUMAN GENOMIC DNA [J].

CHENG, S ;

FOCKLER, C ;

BARNES, WM ;

HIGUCHI, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5695-5699

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