THE CHARACTERIZATION AND CRYSTALLIZATION OF A VIRALLY ENCODED USTILAGO-MAYDIS KP4 TOXIN

被引：9

作者：

GU, F

SULLIVAN, TS

CHE, ZW

GANESA, C

FLURKEY, WH

BOZARTH, RF

SMITH, TJ

机构：

[1] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907

[2] INDIANA STATE UNIV,DEPT LIFE SCI,TERRE HAUTE,IN 47809

[3] INDIANA STATE UNIV,DEPT CHEM,TERRE HAUTE,IN 47809

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 243卷 / 04期

关键词：

FUNGUS; TOXIN; VIRUS; USTILAGO MAYDIS; CRYSTALLIZATION;

D O I：

10.1016/0022-2836(94)90048-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

KP4 is a virally encoded and highly specific toxin that kills fungi closely related to the fungus Ustilago maydis. The toxin was purified and, crystals were formed using ammonium sulfate as precipitant. The crystals belong to the space group P6(1(5))22 and diffracted to similar to 2.2 Angstrom resolution. Circular dicroism spectroscopy suggests that the protein is predominantly comprised of beta-strands.

引用

页码：792 / 795

页数：4

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