BRAIN DISORDERS INDUCED BY PHARMACOLOGICAL BLOCKADE OF PENTOSE PHOSPHATE PATHWAY

6-ANADP synthesized by the endoplasmic glycohydrolase in the brain cells of rats after application of 6-AN is an inhibitor of NADP-dependent dehydrogenases. Under conditions in vivo, 6-phosphogluconate dehydrogenase seems to be the most sensitive enzyme. In the brain, a high accumulation of 6-phosphogluconate and, to a smaller extent, of glucose 6-phosphate was found. The determination of the ratio glucose 6-phosphate: fructose 6-phosphate in the brains of animals pre-treated with 6-AN showed that the accumulation of 6-phosphogluconate also seems to inhibit the PGI in vivo. After application of 14C D-glucose the specific activity of the RNA from cell nuclei of the brain is considerably decreased in animals pretreated with 6-AN. This is supposed to be caused by the inhibition of the oxidative and the non-oxidative part of the pentose phosphate cycle. © 1969.