EVIDENCE THAT THE PHOTOELECTRIC RESPONSE OF BACTERIORHODOPSIN OCCURS IN LESS THAN 5 PICOSECONDS

被引:61
作者
SIMMETH, R
RAYFIELD, GW
机构
[1] Physics Department, University of Oregon, Eugene, Oregon
关键词
D O I
10.1016/S0006-3495(90)82629-1
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The initial photoinduced charge separation in bacteriorhodopsin is shown to occur in <5 ps. This result is obtained by measuring the photovoltage rise time in an oriented film of bacteriorhodopsin (BR). A dye laser syncronously pumped by an Argon ion cw mode locked laser is used to produce 3-ps light pulses which, after passing through a dye amplifier chain, photoexcite the BR sample. The photovoltage transient is detected by an ultra-fast Josephson junction digital sampling oscilloscope with liquid-helium-cooled input circuitry. © 1990, The Biophysical Society. All rights reserved.
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页码:1099 / 1101
页数:3
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