CD4-LIKE MOLECULES IN HUMAN SPERM

被引：36

作者：

GOBERT, B

AMIEL, C

TANG, JQ

BARBARINO, P

BENE, MC

FAURE, G

机构：

[1] Laboratoire d'Immunologie, Faculté de Médecine, Université de Nancy I, Vandoeuvre-les Nancy, 54500

来源：

FEBS LETTERS | 1990年 / 261卷 / 02期

关键词：

CD4; Flow cytometry; Human sperm; Monoclonal antibody; Western blot;

D O I：

10.1016/0014-5793(90)80586-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of a molecule recognized by CD4 monoclonal antibodies was investigated on human sperm using immunolabelling, biochemical and immunochemical methods. Flow cytometry detected a significant fluorescence signal. SDS-PAGE analysis and Western blotting identified a molecule of 60 kDa, consistent with a CD4-like structure as confirmed after selective immunoseparation. Additional bands reacting with anti-CD4 were found in sperm extracts (73 kDa) and seminal fluid (90 kDa). These data indicate that sperm express a molecule similar to the receptor for HIV described on mononuclear cells. © 1990.

引用

页码：339 / 342

页数：4

共 11 条

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ALEXANDER NJ, 1987, FERTIL STERIL, V47, P192

[2]

BAGASRA O, 1988, J ACQ IMMUN DEF SYND, V1, P431

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