DETERMINATION OF THIOSULFATE IN BODY-FLUIDS BY GC AND GC/MS

被引：33

作者：

KAGE, S ^{[1
]}

NAGATA, T ^{[1
]}

KUDO, K ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT FORENS MED,FUKUOKA 812,JAPAN

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1991年 / 15卷 / 03期

关键词：

D O I：

10.1093/jat/15.3.148

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive method to determine thiosulfate in human blood and urine was devised. Thiosulfate was first alkylated with pentafluorobenzyl bromide, with L-ascorbic acid as the stabilizer and sodium chloride as the catalyst. The alkylated thiosulfate was oxidized with iodine to obtain bis(pentafluorobenzyl)disulfide, which was then subjected to gas chromatography and gas chromatography/mass spectrometry. Mass fragmentography was used to identify the disulfide, and gas chromatography with an electron capture detector was used for quantitative determination. The lower limit of detection was 0.003-mu-mol/mL.

引用

页码：148 / 150

页数：3

共 9 条

[1] OXIDATION OF SODIUM SULFIDE BY RAT-LIVER, LUNGS AND KIDNEY [J].