A NEW METHOD OF MEASURING PROTEIN-METHIONINE-S-OXIDE REDUCTASE-ACTIVITY

被引：13

作者：

FERGUSON, DL ^{[1
]}

BURKE, JJ ^{[1
]}

机构：

[1] USDA,PLANT STRESS & WATER CONSERVAT RES UNIT,ROUTE 3,BOX 215,LUBBOCK,TX 79401

来源：

PLANT PHYSIOLOGY | 1992年 / 100卷 / 01期

关键词：

D O I：

10.1104/pp.100.1.529

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A new assay for measuring protein-methionine-S-oxide reductase is reported. The assay measures the conversion of N-(9-fluorenylmethoxycarbonyl)-methionine sulfoxide to N-(9-fluorenylmethoxycarbonyl)-methionine using fluorescence detection after high-performance liquid chromatography separation. Enzyme activity is linear over a 60-min period and the assay is sensitive enough to detect the consumption of only 1% of available substrate. Protein-methionine-S-oxide reductase activity was demonstrated in pea (Pisum sativum L.) chloroplasts, and enzyme levels in leaves of etiolated and light-grown seedlings were compared.

引用

页码：529 / 532

页数：4

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